Meeting Abstract

P3-66   -   Effects of Roundup on Nitrative Stress and Renin Expression in Kidney of Goldfish Noor, MI*; Rahman, MD; University of Texas Rio Grande Valley; University of Texas Rio Grande Valley mdimran.noor01@utrgv.edu

Growing population, urbanization, and heavy anthropogenic activities amplified interaction with the environment, leading to exponential exploitation of natural resources, which often results in environmental pollution. We frequently bombarded aquatic environments with toxic chemicals. One of the significant sources of noxious chemical effluent in the aquatic environment is the ever-growing classification of different pesticides used in agriculture. In our study, we examined the dose-dependent (low dose: 0.5 mg/L, high dose: 5 mg/L) effects of Roundup, a glyphosate-based herbicide, on nitrative stress and renin expression in the kidney of goldfish ( Carassius auratus, a model teleost species). Histopathological analysis showed widespread damage, including fusion of secondary lamellae, long thin filaments like primary lamellae, deterioration of tubular epithelium, rupture of the epithelial layer, reduction in glomerular area, and hemorrhaging in kidney tissues in Roundup exposure groups compared to control. Immunohistochemical analysis demonstrated a significant increase in nitrotyrosine protein (NTP, a biomarker of reactive nitrogen species) and renin expressions in kidney tissues under Roundup exposure groups. Overall, our findings suggest that glyphosate-based herbicide Roundup increases RNS, which leads to damage in kidney tissues as well as impair osmoregulatory functions in teleost species.